Gawrisch, Klaus, Ph.D.
individual record
Senior Investigator
Positions:
- Senior Investigator, Laboratory of Membrane Biochemistry and Biophysics
overview
Dr. Gawrisch was born in Freyburg, Germany in 1950. He received training in biophysics in the laboratory of Lev A. Blumenfeld and a Master's Degree in Physics from Moscow State University, Russia, in 1975. In Leipzig, at the laboratories of Gotthard Klose and Klaus Arnold he was trained in membrane biophysics and Nuclear Magnetic Resonance (NMR) and received a Ph.D. in Physics in 1979 and the Habilitation in Physics in 1986 both from the University of Leipzig, Germany. Until 1989 he served as Docent in Physics and Biophysics at the University of Leipzig. In 1989 he joined the laboratory of Adrian Parsegian at the Division of Computer Research and Technology and later the NMR laboratory of James Ferretti at the National Heart Lung and Blood Institute of the National Institutes of Health in Bethesda, Maryland. From 1993-1998 he was a Tenure Track Investigator heading the Section of NMR of the Laboratory of Membrane Biochemistry and Biophysics at the National Institute on Alcohol Abuse and Alcoholism of NIH. He is currently the Chief of the Laboratory of Membrane Biochemistry and Biophysics and the Chief of the Section of NMR in the laboratory. For his achievements in lipid biophysics he received the 2007 Avanti Award in Lipids of the Biophysical Society.
selected publications
Academic Articles114
- (2018). Stimulation of alpha-synuclein amyloid formation by phosphatidylglycerol micellar tubules. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES. 1860(9), 1840-1847.
- (2017). Expression and NMR Structural Studies of Isotopically Labeled Cannabinoid Receptor Type II. Methods in Enzymology. 593, 387-403.
- (2015). Pore Hydration States of KcsA Potassium Channels in Membranes. JOURNAL OF BIOLOGICAL CHEMISTRY. 290(44), 26765-U472.
- (2015). Rhodopsin/Lipid Hydrophobic Matching-Rhodopsin Oligomerization and Function. BIOPHYSICAL JOURNAL. 108(5), 1125-1132.
- (2014). Global fold of human cannabinoid type 2 receptor probed by solid-state C-13-, N-15-MAS NMR and molecular dynamics simulations. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS. 82(3), 452-465.
- (2014). Structural interactions of a voltage sensor toxin with lipid membranes. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 111(50), E5463-E5470.
- (2014). The Molecular Structure of the Liquid-Ordered Phase of Lipid Bilayers. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 136(2), 725-732.
- (2014). The role of membrane curvature elastic stress for function of rhodopsin-like G protein-coupled receptors. BIOCHIMIE. 107, 28-32.
- (2013). A generalized strategy for immobilizing uniformly oriented membrane proteins at solid interfaces. CHEMICAL COMMUNICATIONS. 49(26), 2685-2687.
- (2013). Application of Halo Tag technology to expression and purification of cannabinoid receptor CB2. PROTEIN EXPRESSION AND PURIFICATION. 89(1), 62-72.
- (2013). Elastic properties of polyunsaturated phosphatidylethanolamines influence rhodopsin function. FARADAY DISCUSSIONS. 161, 383-395.
- (2013). Expression, surface immobilization, and characterization of functional recombinant cannabinoid receptor CB2. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS. 1834(10), 2045-2056.
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- (2013). Simulations of Anionic Lipid Membranes: Development of Interaction-Specific Ion Parameters and Validation Using NMR Data. JOURNAL OF PHYSICAL CHEMISTRY B. 117(35), 10183-10192.
- (2013). Surface plasmon resonance applied to G protein-coupled receptors. Biomedical Spectroscopy and Imaging. 2(3), 155-181.
- (2013). Z and E rotamers of N-formyl-1-bromo-4-hydroxy-3-methoxymorphinan-6-one and their interconversion as studied by H-1/C-13 NMR spectroscopy and quantum chemical calculations. MAGNETIC RESONANCE IN CHEMISTRY. 51(2), 82-88.
- (2012). Recombinant Cannabinoid Type 2 Receptor in Liposome Model Activates G Protein in Response to Anionic Lipid Constituents. JOURNAL OF BIOLOGICAL CHEMISTRY. 287(6), 4076-4087.
- (2012). Structural Interactions between Lipids, Water and S1-S4 Voltage-Sensing Domains. JOURNAL OF MOLECULAR BIOLOGY. 423(4), 632-647.
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- (2012). The role of the lipid matrix for structure and function of the GPCR rhodopsin. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES. 1818(2), 234-240.
- (2011). Structure and Dynamics of Cholesterol-Containing Polyunsaturated Lipid Membranes Studied by Neutron Diffraction and NMR. JOURNAL OF MEMBRANE BIOLOGY. 239(1-2), 63-71.
- (2010). A Lipid Pathway for Ligand Binding Is Necessary for a Cannabinoid G Protein-coupled Receptor. JOURNAL OF BIOLOGICAL CHEMISTRY. 285(23), 17954-17964.
- (2010). Contribution of Membrane Elastic Energy to Rhodopsin Function. BIOPHYSICAL JOURNAL. 99(3), 817-824.
- (2010). Preparation of stable isotope-labeled peripheral cannabinoid receptor CB2 by bacterial fermentation. PROTEIN EXPRESSION AND PURIFICATION. 70(2), 236-247.
- (2009). A layer model of ethanol partitioning into lipid membranes. GENERAL PHYSIOLOGY AND BIOPHYSICS. 28(2), 140-145.
- (2009). Location, Structure, and Dynamics of the Synthetic Cannabinoid Ligand CP-55,940 in Lipid Bilayers. BIOPHYSICAL JOURNAL. 96(12), 4916-4924.
- (2009). Structure and hydration of membranes embedded with voltage-sensing domains. NATURE. 462(7272), 473-U168.
- (2008). Collective and noncollective models of NMR relaxation in lipid vesicles and multilayers. JOURNAL OF PHYSICAL CHEMISTRY B. 112(19), 5924-5929.
- (2008). Insights from biophysical studies on the role of polyunsaturated fatty acids for function of G-protein coupled membrane receptors. PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS. 79(3-5), 131-134.
- (2008). Internal hydration increases during activation of the G-protein-coupled receptor rhodopsin. JOURNAL OF MOLECULAR BIOLOGY. 381(2), 478-486.
- (2008). Lipid-rhodopsin hydrophobic mismatch alters rhodopsin helical content. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 130(37), 12465-12471.
- (2008). Progressive ordering with decreasing temperature of the phospholipids of influenza virus. Nature Chemical Biology. 4(4), 248-255.
- (2008). Structure and dynamics of polyunsaturated hydrocarbon chains in lipid bilayers - significance for GPCR function. CHEMISTRY AND PHYSICS OF LIPIDS. 153(1), 64-75.
- (2007). Biography of Klaus Arnold. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS. 36(4-5), 263-264.
- (2007). Critical fluctuations in domain-forming lipid mixtures. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 104(45), 17650-17655.
- (2007). Docosahexaenoyl chains isomerize on the sub-nanosecond time scale. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 129(21), 6678-+.
- (2007). Expression and purification of CB2 for NMR studies in micellar solution. PROTEIN AND PEPTIDE LETTERS. 14(10), 1031-1037.
- (2007). Hydration of POPC bilayers studied by H-1-PFG-MAS-NOESY and neutron diffraction. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS. 36(4-5), 281-291.
- (2007). Use of dual affinity tags for expression and purification of functional peripheral cannabinoid receptor. PROTEIN EXPRESSION AND PURIFICATION. 53(1), 153-163.
- (2006). Bacterial expression of functional, biotinylated peripheral cannabinoid receptor CB2. PROTEIN EXPRESSION AND PURIFICATION. 49(1), 60-70.
- (2006). Characterization of the liquid-ordered state by proton MAS NMR. BIOPHYSICAL JOURNAL. 90(6), 2051-2061.
- (2006). Closed-loop miscibility gap and quantitative tie-lines in ternary membranes containing diphytanoyl PC. BIOPHYSICAL JOURNAL. 90(12), 4428-4436.
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- (2006). Functional reconstitution of rhodopsin into tubular lipid bilayers supported by nanoporous media. BIOCHEMISTRY. 45(51), 15583-15590.
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- (2006). The structure of polyunsaturated lipid bilayers important for rhodopsin function: A neutron diffraction study. BIOPHYSICAL JOURNAL. 90(1), L4-L6.
- (2005). Diffusion of cholesterol and its precursors in lipid membranes studied by H-1 pulsed field gradient magic angle spinning NMR. BIOPHYSICAL JOURNAL. 89(4), 2504-2512.
- (2005). Electrostatic contributions to indole-lipid interactions. JOURNAL OF PHYSICAL CHEMISTRY B. 109(26), 13014-13023.
- (2005). Expression of human peripheral cannabinoid receptor for structural studies. PROTEIN SCIENCE. 14(10), 2638-2653.
- (2005). Lipid-ethanol interaction studied by NMR on bicelles. JOURNAL OF PHYSICAL CHEMISTRY B. 109(15), 7540-7547.
- (2005). Molecular dynamics investigation of dynamical properties of phosphatidylethanolamine lipid bilayers. JOURNAL OF CHEMICAL PHYSICS. 122(24),
- (2005). Probing specific lipid-protein interaction by saturation transfer difference NMR spectroscopy. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 127(38), 13110-13111.
- (2005). Properties of docosahexaenoic-acid-containing lipids and their influence on the function of rhodopsin. CURRENT OPINION IN STRUCTURAL BIOLOGY. 15(4), 416-422.
- (2005). Specific volumes of unsaturated phosphatidylcholines in the liquid crystalline lamellar phase. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES. 1715(1), 65-70.
- (2004). Domains in binary SOPC/POPE lipid mixtures studied by pulsed field gradient H-1 MAS NMR. BIOPHYSICAL JOURNAL. 87(3), 1741-1751.
- (2004). Liquid domains in vesicles investigated by NMR and fluorescence microscopy. BIOPHYSICAL JOURNAL. 86(5), 2910-2922.
- (2004). Multi-dimensional pulsed field gradient magic angle spinning NMR experiments on membranes. MAGNETIC RESONANCE IN CHEMISTRY. 42(2), 115-122.
- (2004). Multinuclear NMR studies of single lipid bilayers supported in cylindrical aluminum oxide nanopores. LANGMUIR. 20(18), 7711-7719.
- (2004). Structure and fluctuations of charged phosphatidylserine bilayers in the absence of salt. BIOPHYSICAL JOURNAL. 86(3), 1574-1586.
- (2003). Lateral diffusion rates of lipid, water, and a hydrophobic drug in a multilamellar liposome. BIOPHYSICAL JOURNAL. 85(3), 1734-1740.
- (2003). Polyunsaturated docosahexaenoic vs docosapentaenoic acid - Differences in lipid matrix properties from the loss of one double bond. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 125(21), 6409-6421.
- (2003). Rhodopsin exhibits a preference for solvation by polyunsaturated docosohexaenoic acid. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 125(15), 4434-4435.
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- (2002). Novel NMR tools to study structure and dynamics of biomembranes. CHEMISTRY AND PHYSICS OF LIPIDS. 116(1-2), 135-151.
- (2002). Nuclear overhauser enhancement spectroscopy cross-relaxation rates and ethanol distribution across membranes. BIOPHYSICAL JOURNAL. 82(3), 1396-1404.
- (2002). Polyunsaturated fatty acids in lipid bilayers: Intrinsic and environmental contributions to their unique physical properties. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 124(2), 318-326.
- (2002). Polyunsaturated lipids in membrane fusion events. CELLULAR & MOLECULAR BIOLOGY LETTERS. 7(2), 262-264.
- (2001). Dehydration induces lateral expansion of polyunsaturated 18 : 0-22 : 6 phosphatidylcholine in a new lamellar phase. BIOPHYSICAL JOURNAL. 81(2), 969-982.
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- (2001). Simultaneous determination of orientational and order parameter distributions from NMR spectra of partially oriented model membranes. JOURNAL OF MAGNETIC RESONANCE. 149(1), 110-113.
- (2000). Strength of Ca2+ binding to retinal lipid membranes: Consequences for lipid organization. BIOPHYSICAL JOURNAL. 78(6), 3011-3018.
- (1999). Investigation of phospholipid area compression induced by calcium-mediated dextran sulfate interaction. BIOPHYSICAL JOURNAL. 77(2), 879-887.
- (1999). Light scattering characterization of extruded lipid vesicles. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS. 28(3), 187-199.
- (1999). Site-specific deuterium order parameters and membrane-bound behavior of a peptide fragment from the intracellular domain of HIV-1 gp41. BIOCHEMISTRY. 38(19), 6327-6334.
- (1998). Influence of docosahexaenoic acid and cholesterol on lateral lipid organization in phospholipid mixtures. BIOCHEMISTRY. 37(49), 17299-17308.
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- (1998). Why is docosahexaenoic acid essential for nervous system function?. BIOCHEMICAL SOCIETY TRANSACTIONS. 26(3), 365-370.
- (1997). Determining ethanol distribution in phospholipid multilayers with MAS-NOESY spectra. BIOCHEMISTRY. 36(15), 4669-4674.
- (1997). Membrane lateral compressibility determined by NMR and X-ray diffraction: Effect of acyl chain polyunsaturation. BIOPHYSICAL JOURNAL. 73(4), 1954-1966.
- (1997). Water permeability of polyunsaturated lipid membranes measured by O-17 NMR. BIOPHYSICAL JOURNAL. 73(2), 855-864.
- (1996). Bond orientational order, molecular motion, and free energy of high-density DNA mesophases. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 93(9), 4261-4266.
- (1996). Direct measurement of salt-bridge solvation energies using a peptide model system: Implications for protein stability. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 93(7), 2985-2990.
- (1996). Effect of unsaturation on the chain order of phosphatidylcholines in a dioleoylphosphatidylethanolamine matrix. BIOPHYSICAL JOURNAL. 71(1), 274-282.
- (1996). Nuclear magnetic resonance investigation of hydrocarbon chain packing in bilayers of polyunsaturated phospholipids. LIPIDS. 31, S199-S203.
- (1996). Small concentrations of alamethicin induce a cubic phase in bulk phosphatidylethanolamine mixtures. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES. 1278(2), 241-246.
- (1995). EFFECT OF THE CONFORMATION OF A PEPTIDE FROM GP41 ON BINDING AND DOMAIN FORMATION IN MODEL MEMBRANES. MOLECULAR MEMBRANE BIOLOGY. 12(1), 77-82.
- (1995). EFFECTS OF ETHANOL ON LIPID BILAYERS CONTAINING CHOLESTEROL, GANGLIOSIDES, AND SPHINGOMYELIN. BIOCHEMISTRY. 34(27), 8852-8860.
- (1995). H-2 NUCLEAR-MAGNETIC-RESONANCE ORDER-PARAMETER PROFILES SUGGEST A CHANGE OF MOLECULAR SHAPE FOR PHOSPHATIDYLCHOLINES CONTAINING A POLYUNSATURATED ACYL-CHAIN. BIOPHYSICAL JOURNAL. 68(6), 2396-2403.
- (1995). ROLE OF INTERACTIONS AT THE LIPID-WATER INTERFACE FOR DOMAIN FORMATION. MOLECULAR MEMBRANE BIOLOGY. 12(1), 83-88.
- (1995). TRANSLOCATION AND ACTIVATION OF PROTEIN-KINASE-C BY THE PLASMA-CELL TUMOR-PROMOTING ALKANE PRISTANE. CANCER RESEARCH. 55(3), 518-524.
- (1994). DIRECT NMR EVIDENCE FOR ETHANOL BINDING TO THE LIPID-WATER INTERFACE OF PHOSPHOLIPID-BILAYERS. BIOCHEMISTRY. 33(26), 8082-8088.
- (1994). INCREASED ADHESION BETWEEN NEUTRAL LIPID BILAYERS - INTERBILAYER BRIDGES FORMED BY TANNIC-ACID. BIOPHYSICAL JOURNAL. 66(6), 1943-1958.
- (1993). EFFECTS OF FUSOGENIC AGENTS ON MEMBRANE HYDRATION - A DEUTERIUM NUCLEAR-MAGNETIC-RESONANCE APPROACH. Methods in Enzymology. 220, 143-157.
- (1993). INTERACTION OF PEPTIDE FRAGMENT-828-848 OF THE ENVELOPE GLYCOPROTEIN OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-I WITH LIPID BILAYERS. BIOCHEMISTRY. 32(12), 3112-3118.
- (1992). ENERGETICS OF A HEXAGONAL LAMELLAR HEXAGONAL-PHASE TRANSITION SEQUENCE IN DIOLEOYLPHOSPHATIDYLETHANOLAMINE MEMBRANES. BIOCHEMISTRY. 31(11), 2856-2864.
- (1992). MEMBRANE DIPOLE POTENTIALS, HYDRATION FORCES, AND THE ORDERING OF WATER AT MEMBRANE SURFACES. BIOPHYSICAL JOURNAL. 61(5), 1213-1223.