Samelson, Lawrence E., M.D.
individual record
Senior Investigator
Positions:
- Senior Investigator, Laboratory of Cellular and Molecular Biology
overview
Dr. Samelson received his M.D. degree from Yale University in 1977. Following training in internal medicine at the University of Chicago, he carried out postdoctoral research at the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases. He joined the Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development in 1985, became senior investigator in 1988, and chief of the Section on Lymphocyte Signaling and deputy branch chief in 1995. He became chief of the Laboratory of Cellular and Molecular Biology, NCI, in 1999.
selected publications
Academic Articles193
- (2019). Pak1 Kinase Promotes Activated T Cell Trafficking by Regulating the Expression of L-Selectin and. Frontiers in Immunology. 10,
- (2019). TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps. Nature Communications. 10,
- (2018). Cooperative assembly of a four-molecule signaling complex formed upon T cell antigen receptor activation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 115(51), E11914-E11923.
- (2018). Intensity and duration of TCR signaling is limited by p38 phosphorylation of ZAP-70(T293) and destabilization of the signalosome. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 115(9), 2174-2179.
- (2018). Plasma membrane LAT activation precedes vesicular recruitment defining two phases of early T-cell activation. Nature Communications. 9,
- (2018). The Cish SH2 domain is essential for PLC-gamma 1 regulation in TCR stimulated CD8(+) T cells. Scientific Reports. 8,
- (2018). Timed Regulation of 3BP2 Induction Is Critical for Sustaining CD8(+) T Cell Expansion and Differentiation. Cell Reports. 24(5), 1123-1135.
- (2017). Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM. Jove-Journal of Visualized Experiments. (124),
- (2017). Recruitment of calcineurin to the TCR positively regulates T cell activation. NATURE IMMUNOLOGY. 18(2), 196-204.
- (2017). Unexpected Cartilage Phenotype in CD4-Cre-Conditional SOS-Deficient Mice. Frontiers in Immunology. 8,
- (2016). Development of nanoscale structure in LAT-based signaling complexes. JOURNAL OF CELL SCIENCE. 129(24), 4548-4562.
- (2016). Hierarchical nanostructure and synergy of multimolecular signalling complexes. Nature Communications. 7,
- (2016). Microvilli set the stage for T-cell activation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 113(40), 11061-11062.
- (2016). madSTORM: a superresolution technique for large-scale multiplexing at single-molecule accuracy. MOLECULAR BIOLOGY OF THE CELL. 27(22), 3591-3600.
- (2015). Absence of both Sos-1 and Sos-2 in peripheral CD4+T cells leads to PI3K pathway activation and defects in migration. EUROPEAN JOURNAL OF IMMUNOLOGY. 45(8), 2389-2395.
- (2015). Cish actively silences TCR signaling in CD8(+) T cells to maintain tumor tolerance. JOURNAL OF EXPERIMENTAL MEDICINE. 212(12), 2095-2113.
- (2015). Cytoskeletal forces during signaling activation in Jurkat T-cells. MOLECULAR BIOLOGY OF THE CELL. 26(4), 685-695.
- (2015). Enhanced T-cell activation and differentiation in lymphocytes from transgenic mice expressing ubiquitination-resistant 2KR LAT molecules. GENE THERAPY. 22(10), 781-792.
- (2015). The Linker for Activation of T Cells (LAT) Signaling Hub: From Signaling Complexes to Microclusters. JOURNAL OF BIOLOGICAL CHEMISTRY. 290(44), 26422-26429.
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- (2014). In vivo functional mapping of the conserved protein domains within murine Themis1. IMMUNOLOGY AND CELL BIOLOGY. 92(8), 721-728.
- (2013). A Phospholipase C-gamma 1-Independent, RasGRP1-ERK-Dependent Pathway Drives Lymphoproliferative Disease in Linker for Activation of T Cells-Y136F Mutant Mice. JOURNAL OF IMMUNOLOGY. 190(1), 147-158.
- (2013). Automatic sorting of point pattern sets using Minkowski functionals. PHYSICAL REVIEW E. 88(2),
- (2013). Cutting Edge: Cell Surface Linker for Activation of T Cells Is Recruited to Microclusters and Is Active in Signaling. JOURNAL OF IMMUNOLOGY. 190(8), 3849-3853.
- (2013). Enhanced Function of Redirected Human T Cells Expressing Linker for Activation of T Cells That Is Resistant to Ubiquitylation. HUMAN GENE THERAPY. 24(1), 27-37.
- (2013). Functional Redundancy of Sos1 and Sos2 for Lymphopoiesis and Organismal Homeostasis and Survival. MOLECULAR AND CELLULAR BIOLOGY. 33(22), 4562-4578.
- (2013). Multipoint Binding of the SLP-76 SH2 Domain to ADAP Is Critical for Oligomerization of SLP-76 Signaling Complexes in Stimulated T Cells. MOLECULAR AND CELLULAR BIOLOGY. 33(21), 4140-4151.
- (2013). Ras and extracellular signal-regulated kinase signaling in thymocytes and T cells. TRENDS IN IMMUNOLOGY. 34(6), 259-268.
- (2013). Resolving multi-molecular protein interactions by photoactivated localization microscopy. METHODS. 59(3), 261-269.
- (2013). Super-resolution characterization of TCR-dependent signaling clusters. IMMUNOLOGICAL REVIEWS. 251, 21-35.
- (2013). The Ability of Sos1 to Oligomerize the Adaptor Protein LAT Is Separable from Its Guanine Nucleotide Exchange Activity in Vivo. Science Signaling. 6(301),
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- (2012). Deconstructing Ras Signaling in the Thymus. MOLECULAR AND CELLULAR BIOLOGY. 32(14), 2748-2759.
- (2012). Interchangeability of Themis1 and Themis2 in Thymocyte Development Reveals Two Related Proteins with Conserved Molecular Function. JOURNAL OF IMMUNOLOGY. 189(3), 1154-1161.
- (2012). LAT-Independent Erk Activation via Bam32-PLC-gamma 1-Pak1 Complexes: GTPase-Independent Pak1 Activation. MOLECULAR CELL. 48(2), 298-312.
- (2011). Enhanced T-cell signaling in cells bearing linker for activation of T-cell (LAT) molecules resistant to ubiquitylation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 108(7), 2885-2890.
- (2011). Functional Nanoscale Organization of Signaling Molecules Downstream of the T Cell Antigen Receptor. IMMUNITY. 35(5), 705-720.
- (2011). Imaging techniques for assaying lymphocyte activation in action. NATURE REVIEWS IMMUNOLOGY. 11(1), 21-33.
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- (2011). Targeted Sos1 deletion reveals its critical role in early T-cell development. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 108(30), 12407-12412.
- (2010). Cooperative interactions at the SLP-76 complex are critical for actin polymerization. EMBO JOURNAL. 29(14), 2315-2328.
- (2010). The LAT Story: A Tale of Cooperativity, Coordination, and Choreography. Cold Spring Harbor Perspectives in Biology. 2(8),
- (2009). Endocytic events in TCR signaling: focus on adapters in microclusters. IMMUNOLOGICAL REVIEWS. 232, 84-98.
- (2009). Formation of STIM and Orai complexes: puncta and distal caps. IMMUNOLOGICAL REVIEWS. 231, 148-159.
- (2009). Genetic evidence for the role of Erk activation in a lymphoproliferative disease of mice. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 106(34), 14502-14507.
- (2009). Priming the Pump: Adhesion Enhances T Cell Antigen Receptor-Induced Signaling. IMMUNITY. 30(1), 3-5.
- (2008). Bam32: a novel mediator of Erk activation in T cells. INTERNATIONAL IMMUNOLOGY. 20(7), 811-818.
- (2008). Dynamic movement of the calcium sensor STIM1 and the calcium channel Orai1 in activated T-cells: Puncta and distal caps. MOLECULAR BIOLOGY OF THE CELL. 19(7), 2802-2817.
- (2008). Rab35 and its GAP EPI64C in T cells regulate receptor recycling and immunological synapse formation. JOURNAL OF BIOLOGICAL CHEMISTRY. 283(26), 18323-18330.
- (2007). c-Cbl-mediated regulation of LAT-nucleated signaling complexes. MOLECULAR AND CELLULAR BIOLOGY. 27(24), 8622-8636.
- (2006). Oligomerization of signaling complexes by the multipoint binding of GRB2 to both LAT and SOS1. NATURE STRUCTURAL & MOLECULAR BIOLOGY. 13(9), 798-805.
- (2006). Persistence of cooperatively stabilized signaling clusters drives T-cell activation. MOLECULAR AND CELLULAR BIOLOGY. 26(19), 7155-7166.
- (2006). Recruitment and activation of PLC gamma 1 in T cells: a new insight into old domains. EMBO JOURNAL. 25(4), 774-784.
- (2006). T-cell antigen receptor-induced signaling complexes: Internalization via a cholesterol-dependent endocytic pathway. TRAFFIC. 7(9), 1143-1162.
- (2005). Dynamic molecular interactions linking the T cell antigen receptor to the actin cytoskeleton. NATURE IMMUNOLOGY. 6(1), 80-89.
- (2005). Early phosphorylation kinetics of proteins involved in proximal TCR-mediated signaling pathways. JOURNAL OF IMMUNOLOGY. 175(4), 2449-2458.
- (2005). Integration with the human genome of peptide sequences obtained by high-throughput mass spectrometry. GENOME BIOLOGY. 6(1),
- (2005). Mutation of the phospholipase C-gamma 1-binding site of LAT affects both positive and negative thymocyte selection. JOURNAL OF EXPERIMENTAL MEDICINE. 201(7), 1125-1134.
- (2004). Binding specificity of multiprotein signaling complexes is determined by both cooperative interactions and affinity preference. BIOCHEMISTRY. 43(14), 4170-4178.
- (2004). Inactivation of c-Cbl reverses neonatal lethality and T cell developmental arrest of SLP-76-deficient mice. JOURNAL OF EXPERIMENTAL MEDICINE. 200(1), 25-34.
- (2004). LAT: a T lymphocyte adapter protein that couples the antigen receptor to downstream signaling pathways. BIOESSAYS. 26(1), 61-67.
- (2004). Markers for detergent-resistant lipid rafts occupy distinct and dynamic domains in native membranes. MOLECULAR BIOLOGY OF THE CELL. 15(6), 2580-2592.
- (2004). Roles of the proline-rich domain in SLP-76 subcellular localization and T cell function. JOURNAL OF BIOLOGICAL CHEMISTRY. 279(15), 15481-15490.
- (2004). Structural basis for differential recognition of tyrosine-phosphorylated sites in the linker for activation of T cells (LAT) by the adaptor Gads. EMBO JOURNAL. 23(7), 1441-1451.
- (2003). All roads lead to actin: the intimate relationship between TCR signaling and the cytoskeleton. IMMUNOLOGICAL REVIEWS. 191(1), 220-235.
- (2003). CD28 engagement promotes actin polymerization through the activation of the small Rho GTPase Cdc42 in human T cells. JOURNAL OF IMMUNOLOGY. 171(5), 2225-2232.
- (2003). The four distal tyrosines are required for LAT-dependent signaling in Fc epsilon RI-mediated mast cell activation. JOURNAL OF EXPERIMENTAL MEDICINE. 198(5), 831-843.
- (2002). A LAT mutation that inhibits T cell development yet induces lymphoproliferation. SCIENCE. 296(5575), 2040-2043.
- (2002). Fyn kinase initiates complementary signals required for IgE-dependent mast cell degranulation. NATURE IMMUNOLOGY. 3(8), 741-748.
- (2002). Role of Grb2 in EGF-stimulated EGFR internalization. JOURNAL OF CELL SCIENCE. 115(9), 1791-1802.
- (2002). Signal transduction mediated by the T cell antigen receptor: The role of adapter proteins. Annual Review of Immunology. 20, 371-394.
- (2002). T cell receptor ligation induces the formation of dynamically regulated signaling assemblies. JOURNAL OF CELL BIOLOGY. 158(7), 1263-1275.
- (2001). Dynamic actin polymerization drives T cell receptor-induced spreading: A role for the signal transduction adaptor LAT. IMMUNITY. 14(3), 315-329.
- (2001). Knock-in mutation of the distal four tyrosines of linker for activation of T cells blocks murine T cell development. JOURNAL OF EXPERIMENTAL MEDICINE. 194(2), 135-142.
- (2000). Association of Grb2 Gads, and phospholipase C-gamma 1 with phosphorylated LAT tyrosine residues - Effect of LAT tyrosine mutations on T cell antigen receptor-mediated signaling. JOURNAL OF BIOLOGICAL CHEMISTRY. 275(30), 23355-23361.
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- (2000). The role of membrane-associated adaptors in T cell receptor signalling. SEMINARS IN IMMUNOLOGY. 12(1), 35-41.
- (1999). Activation of nuclear factor of activated T cells-(NFAT) and activating protein 1 (AP-1) by oncogenic 70Z Cbl requires an intact phosphotyrosine binding domain but not Crk(L) or p85 phosphatidylinositol S-kinase association. JOURNAL OF BIOLOGICAL CHEMISTRY. 274(8), 5153-5162.
- (1999). Adaptor proteins and T-cell antigen receptor signaling. PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY. 71(3-4), 393-403.
- (1999). Association of the adaptor molecule LAT with CD4 and CD8 coreceptors identifies a new coreceptor function in T cell receptor signal transduction. JOURNAL OF EXPERIMENTAL MEDICINE. 190(10), 1517-1525.
- (1999). Cutting edge: A role for the adaptor protein LAT in human NK cell-mediated cytotoxicity. JOURNAL OF IMMUNOLOGY. 162(5), 2453-2456.
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- (1999). Functional analysis of LAT in TCR-mediated signaling pathways using a LAT-deficient Jurkat cell line. INTERNATIONAL IMMUNOLOGY. 11(6), 943-950.
- (1999). LAT is required for tyrosine phosphorylation of phospholipase C gamma 2 and platelet activation by the collagen receptor GPVI. MOLECULAR AND CELLULAR BIOLOGY. 19(12), 8326-8334.
- (1999). Linker for activation of T cells (LAT), a novel immunohistochemical marker for T cells, NK cells, mast cells, and megakaryocytes - Evaluation in normal and pathological conditions. AMERICAN JOURNAL OF PATHOLOGY. 154(4), 1037-1046.
- (1999). Requirement of the Src homology 2 domain protein Shb for T cell receptor-dependent activation of the interleukin-2 gene nuclear factor for activation of T cells element in Jurkat T cells. JOURNAL OF BIOLOGICAL CHEMISTRY. 274(39), 28050-28057.
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- (1999). The oncogenic 70Z Cbl mutation blocks the phosphotyrosine binding domain-dependent negative regulation of ZAP-70 by c-Cbl in Jurkat T cells. MOLECULAR AND CELLULAR BIOLOGY. 19(10), 6652-6664.
- (1998). Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: Reconstitution studies in a ZAP-70-deficient jurkat T-Cell line. MOLECULAR AND CELLULAR BIOLOGY. 18(3), 1388-1399.
- (1998). LAT is required for TCR-mediated activation of PLC gamma 1 and the Ras pathway. IMMUNITY. 9(5), 617-626.